Compound profile

BPC-157

BPC-157 is a synthetic pentadecapeptide whose sequence corresponds to a partial fragment of a protein characterized in human gastric juice. It is supplied as a reference standard for in-vitro study of cell-migration, fibroblast, and tube-formation (in-vitro angiogenesis) assay systems.

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What it is

BPC-157 is a single-chain, non-glycosylated pentadecapeptide (15 amino acid residues) with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV). 'BPC-157' is the conventional laboratory designation for this peptide; its sequence is derived from, and corresponds to a partial fragment of, a protein characterized in human gastric juice. The catalog material is produced by chemical synthesis rather than biological extraction. It is a stable peptide bearing no disulfide bridges or post-translational modifications, with a free-base molecular formula of C62H98N16O22 and a monoisotopic/average molecular weight of approximately 1419.55 g/mol. It is frequently handled as the acetate salt for improved aqueous solubility. In the laboratory it functions as a defined small-peptide reference compound and is commonly used as a comparator in cell-based assays.

Mechanism (in-vitro research context)

In published in-vitro work, BPC-157 has been described in the context of endothelial signaling rather than binding at a single classical receptor. Studies in cultured human vascular endothelial cells report increased expression of vascular endothelial growth factor receptor 2 (VEGFR2) together with activity along the downstream VEGFR2-Akt-eNOS axis, an effect reported to be sensitive to inhibitors of receptor internalization. Associated readouts in cell culture include endothelial tube formation, ERK1/2 phosphorylation, and greater fibroblast and endothelial cell migration in scratch/wound-closure assays. These observations are confined to in-vitro and cell-based experimental systems and describe biochemical pathway activity in cell culture only; they are not statements of physiological, pharmacological, or therapeutic effect.

Research areas

Endothelial tube-formation (in-vitro angiogenesis) assay systems
Fibroblast and endothelial cell-migration (scratch/wound-closure) assays
VEGFR2-Akt-eNOS signaling-pathway studies in cultured endothelial cells
Nitric oxide (NO) pathway studies in cell-culture models
Extracellular-matrix and collagen-related fibroblast assay systems
Reference-standard and comparator use in peptide analytical chemistry (HPLC/MS)

Chemistry

Molecular weight: 1419.55 g/mol (free base)
CAS: 137525-51-0
Formula: C62H98N16O22 (free base)
Sequence: GEPPPGKPADDAGLV (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val)

Laboratory handling

Supplied lyophilized. For long-term storage, keep the lyophilized powder at -20 °C, protected from light and moisture. Once reconstituted in an appropriate research-grade solvent, store working solutions at 2-8 °C for short-term laboratory use and aliquot for -20 °C storage to minimize freeze-thaw cycles. As an aspartate/proline-containing peptide, it can be susceptible to degradation under elevated temperature and pH extremes; confirm identity and stability analytically (e.g., HPLC and mass spectrometry) before use in an assay.

FAQ

What is the amino acid sequence?

BPC-157 is a pentadecapeptide with the sequence GEPPPGKPADDAGLV (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val), corresponding to a partial fragment of a protein characterized in human gastric juice.

What are the molecular formula and molecular weight?

The free-base molecular formula is C62H98N16O22, with a molecular weight of approximately 1419.55 g/mol. Acetate-salt material will have a correspondingly higher mass due to the counter-ion.

How is purity verified?

Peptide identity and purity are typically confirmed by reversed-phase HPLC for purity quantitation and by mass spectrometry (ESI-MS or MALDI-TOF) for molecular-weight confirmation. Catalog material is specified at a minimum purity of 99% by HPLC.

How should it be stored in the lab?

Store the lyophilized powder at -20 °C, protected from light and moisture. Reconstituted solutions should be kept at 2-8 °C for short-term work and aliquoted for -20 °C storage to limit freeze-thaw cycles, with analytical stability confirmed before use.

What salt form is supplied?

BPC-157 is commonly supplied as the acetate salt, which improves aqueous solubility relative to the free base. The free-base form carries CAS 137525-51-0; the acetate salt is registered under separate CAS numbers.

References

Research-use only

For laboratory research use only. Not for human or veterinary use. Not for diagnostic or therapeutic use. These products have not been approved or evaluated by the U.S. Food and Drug Administration. No statement on this site has been evaluated by the FDA. These products are not intended to diagnose, treat, cure, mitigate, or prevent any disease, and are not intended for human or veterinary use. See our research-use terms and terms of service.