Compound profile

Tesamorelin

Tesamorelin is a synthetic, stabilized 44-residue analog of human growth-hormone-releasing hormone (GHRH). It is supplied strictly as a reference peptide for in-vitro GHRH-receptor binding and signaling research.

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What it is

Tesamorelin is a synthetic peptide corresponding to the full-length human GHRH(1-44) sequence bearing an N-terminal trans-3-hexenoyl modification. This acylation distinguishes it from native GHRH and is associated with increased resistance to proteolytic cleavage relative to the unmodified hormone, making the molecule a useful stabilized analog in biochemical assays. As a member of the GHRH/secretin/glucagon peptide superfamily, it presents the conserved N-terminal pharmacophore required for engagement of the class B G-protein-coupled GHRH receptor (GHRHR). It is characterized as a single, defined peptide entity with a reported average molecular weight of 5135.79 Da and is handled as a lyophilized solid for laboratory research applications.

Mechanism (in-vitro research context)

In in-vitro systems, tesamorelin functions as a ligand for the GHRH receptor (GHRHR), a class B (secretin-family) G-protein-coupled receptor. The conserved N-terminal residues of the GHRH(1-44) sequence constitute the activation domain that engages the receptor's transmembrane core, while C-terminal residues contribute to high-affinity docking at the receptor's extracellular domain. Receptor engagement in cell-based and membrane assays is typically studied through Gs-coupled activation of adenylate cyclase and the resulting accumulation of intracellular cyclic AMP (cAMP), commonly quantified via reporter-gene or competitive immunoassay readouts. The trans-3-hexenoyl N-terminal modification is of interest in stability-focused studies because it alters susceptibility to enzymatic degradation. All characterization described here pertains to receptor-pharmacology and signal-transduction endpoints measured in vitro.

Research areas

GHRH-receptor (GHRHR) radioligand and fluorescent-ligand binding assays
Gs/adenylate-cyclase coupling and intracellular cAMP accumulation in receptor-expressing cell lines
Class B GPCR structure-activity and pharmacophore-mapping studies
Comparative enzymatic-stability and proteolytic-degradation profiling versus native GHRH(1-44)
Analytical method development (RP-HPLC, LC-MS) for peptide identity and purity confirmation

Chemistry

Molecular weight: 5135.79 g/mol (average)
CAS: 218949-48-5
Formula: C221H366N72O67S
Sequence: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu (GHRH 1-44) with N-terminal trans-3-hexenoyl modification

Laboratory handling

Store the lyophilized peptide at -20 °C, protected from light and moisture, where it remains stable under these conditions. For laboratory use, dissolve in a suitable laboratory-grade aqueous or buffered solvent; store working solutions at 2-8 °C for short-term laboratory use and aliquot for storage at -20 °C to limit repeated freeze-thaw cycles. As a multi-residue peptide containing methionine, minimize prolonged exposure to oxidative conditions and elevated temperatures. Confirm identity, purity, and integrity analytically (e.g., RP-HPLC and mass spectrometry) before experimental use.

FAQ

What is the amino acid sequence?

Tesamorelin corresponds to the full human GHRH(1-44) sequence (Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu) carrying an N-terminal trans-3-hexenoyl group that distinguishes it from native GHRH.

What is the molecular weight and formula?

The reported average molecular weight is 5135.79 Da, with a molecular formula of C221H366N72O67S.

What is its molecular target in receptor assays?

It is studied as a ligand for the GHRH receptor (GHRHR), a class B G-protein-coupled receptor, typically via Gs/adenylate-cyclase coupling and cAMP-accumulation readouts in receptor-expressing cells.

How is purity verified?

Identity and purity are commonly confirmed by reversed-phase HPLC for purity quantitation and by mass spectrometry (e.g., ESI-MS) for molecular-weight confirmation; a typical research-grade specification is greater than or equal to 98 percent by HPLC.

How should it be stored in the lab?

Keep the lyophilized solid at -20 °C, protected from light and moisture. Prepare working solutions in a suitable laboratory-grade solvent, store short-term at 2-8 °C, and aliquot for -20 °C storage to avoid repeated freeze-thaw cycles; verify stability analytically before use.

Research-use only

For laboratory research use only. Not for human or veterinary use. Not for diagnostic or therapeutic use. These products have not been approved or evaluated by the U.S. Food and Drug Administration. No statement on this site has been evaluated by the FDA. These products are not intended to diagnose, treat, cure, mitigate, or prevent any disease, and are not intended for human or veterinary use. See our research-use terms and terms of service.